ISM is a super-resolution imaging technique that enhances resolution beyond the diffraction limit through pixel reassignment and deconvolution. However, the practical implementation of ISM requires ...

What is the Diffraction Limit? The diffraction limit is a fundamental barrier in optical microscopy that sets the minimum size of features that can be resolved using conventional light microscopes. It ...

Fluorescence microscopy techniques are powerful tools for probing very small signals and revealing three-dimensional (3D) structural and functional properties of biological samples with high ...