The LUX primer method (based on the formation of hairpin structures within the primer to bring nearby G or C bases into close proximity to a fluorophore attached to a T base, to quench its ...

The polymerase chain reaction (PCR) is a molecular method that is employed for in vitro amplification of a specific region in a strand of deoxyribonucleic acid (DNA). Multiplex PCR involves the ...

The changing technology landscape will affect the BioWatch program. This chapter comments on additional assay performance considerations that arise from the use of multiplexed real-time polymerase ...

In vitro diagnostics (IVD) is an umbrella term for tests conducted on blood or tissue samples to detect diseases, determine the efficacy of novel or established treatments, and monitor health.

Multiplex PCR is a difficult technique that allows amplification of two or more products in parallel in a single reaction tube. This technology was first described by Chamberlain et al., in 1988, and ...

The modular multiplex RT-PCR method simultaneously detects Shiga Toxin E. coli (STEC), Salmonella spp. and Listeria monocytogenes using a single, overnight enrichment (24 hours). Ready-to-use PCR ...

Molecular analysis using PCR techniques demands both precision and versatility, integrating methods that offer unique yet complementary strengths. However, researchers can face challenges balancing ...

In this Tech Blast episode, we discuss multiplex PCR methods, their challenges and advantages, and the tools being developed to advance multiplexing capabilities. Marco Bianchi, Product Manager at Bio ...

Want to share this FREE webinar with a colleague? Fill out the form below and we'll send your colleague an invitation to the "Multiplex qPCR: Techniques and Advantages for Gene Expression Studies ...

As a prerequisite to use the QIAcuity High Multiplex Probe PCR Kit, the QIAcuity Software 3.1 introduces essential features for high-order multiplexing such as crosstalk compensation which corrects ...

Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics. As a sensitive and precise method for quantitating specific nucleic acid targets, qPCR is ...